Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9905] to BOG
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-BOG antibody [EPR9905]
See all BOG primary antibodies
Description

Rabbit monoclonal [EPR9905] to BOG
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human BOG. The exact sequence is proprietary.
Database link: O75884
Positive control
- HepG2, MCF7, and HeLa cell lysates; kidney tissue; MCF7 and HeLa cells.
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Previously labelled as RBBP9.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9905
Isotype

IgG
Research areas

Western blot - Anti-BOG antibody [EPR9905] (ab157202)

All lanes : Anti-BOG antibody [EPR9905] (ab157202) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOG antibody [EPR9905] (ab157202)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling BOG using ab157202 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [EPR9905] (ab157202)

Immunofluorescence analysis of MCF7 cells, labeling BOG using ab157202 at a 1/250 dilution.
Flow Cytometry - Anti-BOG antibody [EPR9905] (ab157202)

Flow cytometry analysis of permeabilized HeLa cells labeling BOG (red), using ab157202 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)
Anti-BOG antibody [EPR9905] (ab157202)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com