Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Thursday February 25, 2021

HRP Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] (ab202879)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EP1857Y] to Nucleophosmin (phospho T199)
Suitable for: Dot blot, WB
Reacts with: Human
Conjugation: HRP

Product name

HRP Anti-Nucleophosmin (phospho T199) antibody [EP1857Y]
See all Nucleophosmin primary antibodies
Description

HRP Rabbit monoclonal [EP1857Y] to Nucleophosmin (phospho T199)
Host species

Rabbit
Conjugation

HRP
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293 treated with Calyculin A.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Monoclonal
Clone number

EP1857Y
Isotype

IgG
Research areas

Western blot - HRP Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] (ab202879)

All lanes : HRP Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] (ab202879) at 1/50000 dilution

Lane 1 : Untreated HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) treated with 0.1 µM Calyculin A for 60 minutes whole cell lysates
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) treated with 0.1 µM Calyculin A for 60 minutes whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.

Lysates/proteins at 15 µg per lane.

Predicted band size: 33 kDa
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 1 second

Blocking buffer: 5% NFDM/TBST

Diluting buffer: 5% NFDM/TBST
Dot Blot - HRP Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] (ab202879)

ab202879 binding Nucleophosmin (phospho T199) in Dot blot at a dilution of 1/1000.

Lane 1: Nucleophosmin phospho T199 peptide

Lane 2: Nucleophosmin non-phospho peptide

Exposure time: 10 seconds

Diluting buffer: 5%NFDM/TBST

Blocking buffer: 5%NFDM/TBST
HRP Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] (ab202879)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com