Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (ab208015)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20172] to Nucleophosmin (citrulline R196)
Suitable for: WB, Dot blot, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Nucleophosmin (citrulline R196) antibody [EPR20172]
See all Nucleophosmin primary antibodies
Description

Rabbit monoclonal [EPR20172] to Nucleophosmin (citrulline R196)
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Recombinant fragment

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: NIH/3T3 transfected with GFP-tagged PADI4 (WT) expression vector, treated with calcium chloride and Ionomycin whole cell lysate; HEK-293T transfected with GFP-tagged PADI2 (WT) expression vector, treated with calcium chloride and Ionomycin whole cell lysate; C6 transfected with GFP-tagged PADI4 (WT) expression vector, treated with calcium chloride and Ionomycin whole cell lysate. IP: HEK 293T transfected with GFP-tagged PADI4 expression vector then treated with CaCl2 and ionomycin whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20172
Isotype

IgG
Research areas

Western blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (ab208015)

All lanes : Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (ab208015) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 2 : NIH/3T3 transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Predicted band size: 32 kDa
Observed band size: 37 kDa
why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
Western blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (ab208015)

All lanes : Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (ab208015) at 1/1000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 2 : HEK-293T transfected with GFP-tagged PADI2 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 3 : C6 (rat glial tumor cell line) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 4 : C6 transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate

Lysates/proteins at 20 µg per lane.

Developed using the ECL technique.

Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?

Exposure time: Lane 1 and 2: 5 seconds; Lane 3 and 4: 3 minutes.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (ab208015)

Nucleophosmin (citrulline R196) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 expression vector for 24h then treated with 10 mM CaCl₂ and 10 µM ionomycin for 2h, whole cell lysate with ab208015 at 1/30 dilution. Western blot was perfromed from the immunoprecipitate using ab208015 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution.

Lane 1: HEK-293T transfected with GFP-tagged PADI4 expression vector for 24h then treated with 10 mM CaCl₂ and 10 µM ionomycin for 2h, whole cell lysate 10μg (Input).

Lane 2: ab208015 IP in HEK-293T transfected with GFP-tagged PADI4 expression vector for 24h then treated with 10 mM CaCl₂ and 10 µM ionomycin for 2h, whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208015 in HEK-293T transfected with GFP-tagged PADI4 expression vector for 24h then treated with 10mM CaCl₂ and 10 µM ionomycin for 2h, whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST

Exposure time : lesss than 1 second.
Dot Blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (ab208015)

Dot blot analysis of Nucleophosmin (citruline R196) labeled with ab208015 at 1/1000 dilution.

Lane 1: Nucleophosmin (citrulline R196) peptide.

Lane 2: Nucleophosmin non-citrulline peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (ab208015)

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