Anti-eIF4EBP1 (phospho T45) antibody (ab47367)
Key features and details
- Rabbit polyclonal to eIF4EBP1 (phospho T45)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-eIF4EBP1 (phospho T45) antibody
See all eIF4EBP1 primary antibodies -
Description
Rabbit polyclonal to eIF4EBP1 (phospho T45) -
Host species
Rabbit -
Specificity
ab47367 detects endogenous levels of 4EBP1 only when phosphorylated at threonine 45. This antibody may cross-react with 4EBP2 and 4EBP3 when phosphorylated at equivalent sites. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human 4EBP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).
-
Positive control
- MDA435 cells, breast carcinoma tissue
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 (phospho T45) antibody (ab47367)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47367. Left hand image without immunising peptide; right hand image with immunising peptide.
-
Western blot - Anti-eIF4EBP1 (phospho T45) antibody (ab47367)Western blot analysis of extracts from MDA435 cells untreated (lane 2) or treated (lane 1) with EGF (200nm, 5min), using ab47367.
