Anti-EEF2K (phospho S366) antibody (ab51227)
Key features and details
- Rabbit polyclonal to EEF2K (phospho S366)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EEF2K (phospho S366) antibody
See all EEF2K primary antibodies -
Description
Rabbit polyclonal to EEF2K (phospho S366) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of EEF2K only when phosphorylated at serine 366. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
A synthesized phosphopeptide derived from human EEF2K around the phosphorylation site of serine 366 (T-L-SP-G-S).
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Positive control
- Hela cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EEF2K (phospho S366) antibody (ab51227)All lanes : Anti-EEF2K (phospho S366) antibody (ab51227) at 1/300 dilution
Lane 1 : Extracts of Hela cells treated with 10% serum for 15mins.
Lane 2 : Extracts of Hela cells treated with 10% serum for 15mins and phosphopeptide.
Predicted band size: 95 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S366) antibody (ab51227)Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab51227 (right picture is blocked with the phospho peptide)
