Anti-EEF2K antibody [EP724Y] (ab46787)
![Anti-EEF2K antibody [EP724Y] (ab46787)](https://www.abcam.com/ps/products/46/ab46787/Images/ab46787-260961-anti-eef2k-antibody-ep724y-western-blot.jpg "Anti-EEF2K antibody [EP724Y] (ab46787)")
Key features and details
- Rabbit monoclonal [EP724Y] to EEF2K
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EEF2K antibody [EP724Y]
See all EEF2K primary antibodies -
Description
Rabbit monoclonal [EP724Y] to EEF2K -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human EEF2K aa 550-650 (internal sequence). The exact sequence is proprietary.
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Positive control
- HeLa and Jurkat cell lysates
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP724Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EEF2K antibody [EP724Y] (ab46787)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: EEF2K knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab46787 observed at 95 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab46787 was shown to specifically react with EEF2K when EEF2K knockout samples were used. Wild-type and EEF2K knockout samples were subjected to SDS-PAGE. ab46787 and ab8245 (loading control to GAPDH) were diluted at 1/50 000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-EEF2K antibody [EP724Y] (ab46787)](https://www.abcam.com/ps/products/46/ab46787/Images/ab46787-59010-anti-eef2k-antibody-ep724y-western-blot.jpg)
Western blot - Anti-EEF2K antibody [EP724Y] (ab46787)All lanes : Anti-EEF2K antibody [EP724Y] (ab46787) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 82 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
