Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Thursday March 04, 2021

Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2252(N)] to EEF2K (phospho S359)
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-EEF2K (phospho S359) antibody [EPR2252(N)]
See all EEF2K primary antibodies
Description

Rabbit monoclonal [EPR2252(N)] to EEF2K (phospho S359)
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa cells, treated with Okadaic Acid + Calyculin A; Human liver and stomach tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2252(N)
Isotype

IgG
Research areas

Western blot - Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)

All lanes : Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257) at 1/1000 dilution

Lane 1 : HeLa cells, treated with Okadaic Acid + Calyculin A
Lane 2 : HeLa cells, untreated

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 82 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling EEF2K with ab192257 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling EEF2K with ab192257 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com