Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)
![Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)](https://www.abcam.com/ps/products/192/ab192257/Images/ab192257-229905-anti-eef2k-phospho-s359-antibody-epr2252n-western-blot.jpg "Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2252(N)] to EEF2K (phospho S359)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-EEF2K (phospho S359) antibody [EPR2252(N)]
See all EEF2K primary antibodies -
Description
Rabbit monoclonal [EPR2252(N)] to EEF2K (phospho S359) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cells, treated with Okadaic Acid + Calyculin A; Human liver and stomach tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2252(N) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)All lanes : Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257) at 1/1000 dilution
Lane 1 : HeLa cells, treated with Okadaic Acid + Calyculin A
Lane 2 : HeLa cells, untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 82 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)](https://www.abcam.com/ps/products/192/ab192257/Images/ab192257-229904-anti-eef2k-phospho-s359-antibody-epr2252n-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling EEF2K with ab192257 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)](https://www.abcam.com/ps/products/192/ab192257/Images/ab192257-229903-anti-eef2k-phospho-s359-antibody-epr2252n-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling EEF2K with ab192257 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)](https://www.abcam.com/ps/products/192/ab192257/Images/ab192257-4-benefits-of-recombinant-antibodies.png)
Anti-EEF2K (phospho S359) antibody [EPR2252(N)] (ab192257)
