Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [OTI3C4] to SNX8
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human, African green monkey
Isotype: IgG2a

Product name

Anti-SNX8 antibody [OTI3C4]
Description

Mouse monoclonal [OTI3C4] to SNX8
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	African green monkey
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human SNX8 aa 1 to the C-terminus. NP_037453. Produced in HEK-293T cells.
Database link: Q9Y5X2

Run BLAST with Run BLAST with
Positive control
- WB: HeLa, A549, COS-7, Jurkat and MCF7 extracts. HEK-293T cell lysate transfected with pCMV6-ENTRY SNX8 cDNA. IHC-P: Human breast adenocarcinoma, kidney, liver, bladder carcinoma and tonsil tissue. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY SNX8 cDNA. Flow Cyt: Jurkat cells.
General notes

Clone OTI3C4 (formerly 3C4).

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography.
Clonality

Monoclonal
Clone number

OTI3C4
Isotype

IgG2a
Research areas

Western blot - Anti-SNX8 antibody [OTI3C4] (ab119388)

All lanes : Anti-SNX8 antibody [OTI3C4] (ab119388) at 1/200 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts
Lane 2 : A549 (Human lung carcinoma cell line) extracts
Lane 3 : COS-7 (African green monkey kidney fibroblast-like cell line) extracts
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) extracts
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) extracts

Lysates/proteins at 10 µg per lane.

Predicted band size: 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

Paraffin-embedded human breast adenocarcinoma tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-SNX8 antibody [OTI3C4] (ab119388)

pCMV6-ENTRY SNX8 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for SNX8 using ab119388 at a 1/100 dilution in ICC/IF.
Flow Cytometry - Anti-SNX8 antibody [OTI3C4] (ab119388)

Flow Cytometry analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells, labeling SNX8 with ab119388 at a 1/100 dilution (red) compared to a non-specific negative control antibody (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

Paraffin-embedded human kidney tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

Paraffin-embedded human liver tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

Paraffin-embedded human bladder carcinoma tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

Paraffin-embedded human tonsil tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Western blot - Anti-SNX8 antibody [OTI3C4] (ab119388)

All lanes : Anti-SNX8 antibody [OTI3C4] (ab119388) at 1/200 dilution

Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY SNX8 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 53 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com