Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SNX8 antibody [OTI3C4] (ab119388)

Key features and details

  • Mouse monoclonal [OTI3C4] to SNX8
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human, African green monkey
  • Isotype: IgG2a

Overview

  • Product name

    Anti-SNX8 antibody [OTI3C4]

  • Description

    Mouse monoclonal [OTI3C4] to SNX8

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    African green monkey
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human SNX8 aa 1 to the C-terminus. NP_037453. Produced in HEK-293T cells.
    Database link: Q9Y5X2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa, A549, COS-7, Jurkat and MCF7 extracts. HEK-293T cell lysate transfected with pCMV6-ENTRY SNX8 cDNA. IHC-P: Human breast adenocarcinoma, kidney, liver, bladder carcinoma and tonsil tissue. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY SNX8 cDNA. Flow Cyt: Jurkat cells.

  • General notes

    Clone OTI3C4 (formerly 3C4).

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Purified from cell culture supernatant by affinity chromatography.

  • Clonality

    Monoclonal

  • Clone number

    OTI3C4

  • Isotype

    IgG2a

  • Research areas

Images

  • Western blot - Anti-SNX8 antibody [OTI3C4] (ab119388)
    Western blot - Anti-SNX8 antibody [OTI3C4] (ab119388)
    All lanes : Anti-SNX8 antibody [OTI3C4] (ab119388) at 1/200 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts
    Lane 2 : A549 (Human lung carcinoma cell line) extracts
    Lane 3 : COS-7 (African green monkey kidney fibroblast-like cell line) extracts
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) extracts
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) extracts

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 53 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

    Paraffin-embedded human breast adenocarcinoma tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunocytochemistry/ Immunofluorescence - Anti-SNX8 antibody [OTI3C4] (ab119388)
    Immunocytochemistry/ Immunofluorescence - Anti-SNX8 antibody [OTI3C4] (ab119388)

    pCMV6-ENTRY SNX8 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for SNX8 using ab119388 at a 1/100 dilution in ICC/IF.

  • Flow Cytometry - Anti-SNX8 antibody [OTI3C4] (ab119388)
    Flow Cytometry - Anti-SNX8 antibody [OTI3C4] (ab119388)

    Flow Cytometry analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells, labeling SNX8 with ab119388 at a 1/100 dilution (red) compared to a non-specific negative control antibody (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

    Paraffin-embedded human kidney tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

    Paraffin-embedded human liver tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

    Paraffin-embedded human bladder carcinoma tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

    Paraffin-embedded human tonsil tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Western blot - Anti-SNX8 antibody [OTI3C4] (ab119388)
    Western blot - Anti-SNX8 antibody [OTI3C4] (ab119388)
    All lanes : Anti-SNX8 antibody [OTI3C4] (ab119388) at 1/200 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY SNX8 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 53 kDa

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