Antibodies, Proteins, Kits and Reagents for Life Science

264 681 ₸ Product size

100 µl 264 681 ₸

Order now and get it on Tuesday March 09, 2021

Anti-TMUB1 antibody [EPR14066] (ab180586)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14066] to TMUB1
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-TMUB1 antibody [EPR14066]
See all TMUB1 primary antibodies
Description

Rabbit monoclonal [EPR14066] to TMUB1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal liver tissue lysate, Human cerebellum tissue lysate, HeLa cells, HepG2 cells, Human liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR14066
Isotype

IgG
Research areas

Western blot - Anti-TMUB1 antibody [EPR14066] (ab180586)

All lanes : Anti-TMUB1 antibody [EPR14066] (ab180586) at 1/10000 dilution

Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : TMUB1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 26 kDa
Observed band size: 27 kDa
why is the actual band size different from the predicted?

Lanes 1- 3: Merged signal (red and green). Green - ab180586 observed at 27 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab180586 was shown to react with TMUB1 in wild-type HeLa (Human epithelial line from cervix adenocarcinoma) cells in western blot. Loss of signal was observed when knockout cell line ab265852 (knockout cell lysate ab258237) was used. Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) and TMUB1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180586 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-TMUB1 antibody [EPR14066] (ab180586)

Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling TMUB1 with ab180586 at 1/70 (pink). Control shown in green.
Immunocytochemistry/ Immunofluorescence - Anti-TMUB1 antibody [EPR14066] (ab180586)

Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cells labeling TMUB1 with ab180586 at 1/500 (green). Dapi staining shown in blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMUB1 antibody [EPR14066] (ab180586)

Immunohistochemical analysis of paraffin embedded Human liver tissue labeling TMUB1 with ab180586 at 1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-TMUB1 antibody [EPR14066] (ab180586)

All lanes : Anti-TMUB1 antibody [EPR14066] (ab180586) at 1/50000 dilution

Lane 1 : Human fetal liver tissue lysate at 20 µg
Lane 2 : Human cerebellum tissue lysate at 20 µg
Lane 3 : HepG2 cell line lysate lysate at 20 µg
Lane 4 : HeLa cell line lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa (possible cleavage fragment)
Anti-TMUB1 antibody [EPR14066] (ab180586)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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