Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TMUB1 antibody [EPR14066] (ab180586)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14066] to TMUB1
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-TMUB1 antibody [EPR14066]
    See all TMUB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14066] to TMUB1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver tissue lysate, Human cerebellum tissue lysate, HeLa cells, HepG2 cells, Human liver tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14066

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TMUB1 antibody [EPR14066] (ab180586)
    Western blot - Anti-TMUB1 antibody [EPR14066] (ab180586)
    All lanes : Anti-TMUB1 antibody [EPR14066] (ab180586) at 1/10000 dilution

    Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : TMUB1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 27 kDa
    why is the actual band size different from the predicted?



    Lanes 1- 3: Merged signal (red and green). Green - ab180586 observed at 27 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab180586 was shown to react with TMUB1 in wild-type HeLa (Human epithelial line from cervix adenocarcinoma) cells in western blot. Loss of signal was observed when knockout cell line ab265852 (knockout cell lysate ab258237) was used. Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) and TMUB1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180586 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-TMUB1 antibody [EPR14066] (ab180586)
    Flow Cytometry - Anti-TMUB1 antibody [EPR14066] (ab180586)

    Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling TMUB1 with ab180586 at 1/70 (pink). Control shown in green.

  • Immunocytochemistry/ Immunofluorescence - Anti-TMUB1 antibody [EPR14066] (ab180586)
    Immunocytochemistry/ Immunofluorescence - Anti-TMUB1 antibody [EPR14066] (ab180586)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cells labeling TMUB1 with ab180586 at 1/500 (green). Dapi staining shown in blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMUB1 antibody [EPR14066] (ab180586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMUB1 antibody [EPR14066] (ab180586)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labeling TMUB1 with ab180586 at 1/100.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-TMUB1 antibody [EPR14066] (ab180586)
    Western blot - Anti-TMUB1 antibody [EPR14066] (ab180586)
    All lanes : Anti-TMUB1 antibody [EPR14066] (ab180586) at 1/50000 dilution

    Lane 1 : Human fetal liver tissue lysate at 20 µg
    Lane 2 : Human cerebellum tissue lysate at 20 µg
    Lane 3 : HepG2 cell line lysate lysate at 20 µg
    Lane 4 : HeLa cell line lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?
    Additional bands at: 24 kDa (possible cleavage fragment)

  • Anti-TMUB1 antibody [EPR14066] (ab180586)
    Anti-TMUB1 antibody [EPR14066] (ab180586)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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