Anti-Toca-1 antibody (ab241299)
Key features and details
- Rabbit polyclonal to Toca-1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Toca-1 antibody
See all Toca-1 primary antibodies -
Description
Rabbit polyclonal to Toca-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Toca-1 aa 450-500. The exact sequence is proprietary. (NP_001157945.1)
Database link: Q5T0N5 -
Positive control
- WB: Jurkat, HEK-293T and HeLa whole cell lysates. IP: Jurkat whole cell lysate. IHC-P: Human lung carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Toca-1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Toca-1 antibody (ab241299)All lanes : Anti-Toca-1 antibody (ab241299) at 0.1 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 70 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-Toca-1 antibody (ab241299)Toca-1 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241299 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different antibody to Toca-1 that recognises an upstream epitope at 1 µg/ml.
Lane 1: ab241299 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Toca-1 antibody (ab241299)Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for Toca-1 using ab241299 at 1/1000 dilution in immunohistochemical analysis. DAB staining.
