Anti-EEF2K antibody (ab96685)
Key features and details
- Rabbit polyclonal to EEF2K
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-EEF2K antibody
See all EEF2K primary antibodies -
Description
Rabbit polyclonal to EEF2K -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human EEF2K aa 1-238.
Database link: O00418 -
Positive control
- ICC/IF: A431 cells. IHC-P: BT483 xenograft. WB: A431 , H1299 , HeLa , HepG2 cells. Mouse liver lysate. PC-12 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin embedded Mouse BT483 xenograft tissue stained for eEF-2K using ab96685 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-EEF2K antibody (ab96685) at 1/5000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 82 kDa
7.5% SDS-PAGE -
ab96685, at 1/200 dilution staining EEF2K in paraformaldehyde-fixed A431 cells by Immunofluorescence. Lower image is merged with DNA probe.
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Anti-EEF2K antibody (ab96685) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 82 kDa7.5% SDS PAGE
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Anti-EEF2K antibody (ab96685) at 1/500 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Predicted band size: 82 kDa7.5% SDS-PAGE