Anti-EEF2K antibody [EP881Y] (ab45168)
![Anti-EEF2K antibody [EP881Y] (ab45168)](https://www.abcam.com/ps/products/45/ab45168/Images/ab45168-260960-anti-eef2k-antibody-ep881y-western-blot.jpg "Anti-EEF2K antibody [EP881Y] (ab45168)")
Key features and details
- Rabbit monoclonal [EP881Y] to EEF2K
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EEF2K antibody [EP881Y]
See all EEF2K primary antibodies -
Description
Rabbit monoclonal [EP881Y] to EEF2K -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human EEF2K aa 700-800 (C terminal). The exact sequence is proprietary.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP881Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EEF2K antibody [EP881Y] (ab45168)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: EEF2K knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab45168 observed at 95 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab45168 was shown to specifically react with EEF2K when EEF2K knockout samples were used. Wild-type and EEF2K knockout samples were subjected to SDS-PAGE. ab45168 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-EEF2K antibody [EP881Y] (ab45168)](https://www.abcam.com/ps/products/45/ab45168/Images/ab45168-57329-anti-eef2k-antibody-ep881y-western-blot.jpg)
Western blot - Anti-EEF2K antibody [EP881Y] (ab45168)Anti-EEF2K antibody [EP881Y] (ab45168) at 1/1000 dilution + HEK293 cell lysate
Predicted band size: 82 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
