Anti-Tomosyn antibody (ab41271)
Key features and details
- Rabbit polyclonal to Tomosyn
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Tomosyn antibody
See all Tomosyn primary antibodies -
Description
Rabbit polyclonal to Tomosyn -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human Tomosyn.
Read Abcam's proprietary immunogen policy (Peptide available as ab45226.) -
Positive control
- WB: brain tissue lysate IHC: brain (hippocampus)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB RatAll applications MouseCowHumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 122,128 kDa (predicted molecular weight: 128 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 122,128 kDa (predicted molecular weight: 128 kDa).Target
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Relevance
Tomosyn plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release by inhibiting the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane, via the displacement of munc18 from syntaxin-1. There are three splice variants of tomosyn designated m-tomosyn, b-tomosyn and s-tomosyn. Although b-tomosyn is ubiquitously expressed, s-tomosyn and m-tomosyn are expressed primarily in brain. -
Cellular localization
Cell Membrane and Cytoplasmic. Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles -
Database links
- Entrez Gene: 134957 Human
- Entrez Gene: 78808 Mouse
- Entrez Gene: 81022 Rat
- Omim: 604586 Human
- SwissProt: Q5T5C0 Human
- SwissProt: Q8K400 Mouse
- SwissProt: Q9WU70 Rat
- Unigene: 93534 Human
see all -
Alternative names
- FLJ30922 antibody
- Lethal(2) giant larvae protein homolog 3 antibody
- LGL3 antibody
see all
Images
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Western blot - Anti-Tomosyn antibody (ab41271)Anti-Tomosyn antibody (ab41271) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 128 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Tamosyn is phosphorylated on a number of serine and threonine residues (SwissProt), which may account for the banding pattern observed. ab41271 is targeted against all isoforms of the Tamosyn protein.
Protocols
Datasheets and documents
References (1)
ab41271 has been referenced in 1 publication.
- Takeuchi S et al. Tomosyn Negatively Regulates Arginine Vasopressin Secretion in Embryonic Stem Cell-Derived Neurons. PLoS One 11:e0164544 (2016). PubMed: 27732637
Images
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Western blot - Anti-Tomosyn antibody (ab41271)Anti-Tomosyn antibody (ab41271) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 128 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Tamosyn is phosphorylated on a number of serine and threonine residues (SwissProt), which may account for the banding pattern observed. ab41271 is targeted against all isoforms of the Tamosyn protein.
