Anti-Tomosyn antibody (ab45228)
Key features and details
- Rabbit polyclonal to Tomosyn
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Tomosyn antibody
See all Tomosyn primary antibodies -
Description
Rabbit polyclonal to Tomosyn -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic peptide corresponding to Human Tomosyn aa 850-950 conjugated to keyhole limpet haemocyanin.
(Peptide available asab45227) -
Positive control
- This antibody gave a positive signal in the following tissues lysates: Rat Brain, Mouse Brain and Human Brain
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB RatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).Target
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Relevance
Tomosyn plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release by inhibiting the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane, via the displacement of munc18 from syntaxin-1. There are three splice variants of tomosyn designated m-tomosyn, b-tomosyn and s-tomosyn. Although b-tomosyn is ubiquitously expressed, s-tomosyn and m-tomosyn are expressed primarily in brain. -
Cellular localization
Cell Membrane and Cytoplasmic. Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles -
Database links
- Entrez Gene: 134957 Human
- Entrez Gene: 78808 Mouse
- Entrez Gene: 81022 Rat
- Omim: 604586 Human
- SwissProt: Q5T5C0 Human
- SwissProt: Q8K400 Mouse
- SwissProt: Q9WU70 Rat
- Unigene: 93534 Human
see all -
Alternative names
- FLJ30922 antibody
- Lethal(2) giant larvae protein homolog 3 antibody
- LGL3 antibody
see all
Images
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Western blot - Anti-Tomosyn antibody (ab45228)All lanes : Anti-Tomosyn antibody (ab45228) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 128 kDa
Additional bands at: 30 kDa, 52 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
References (0)
ab45228 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Tomosyn antibody (ab45228)All lanes : Anti-Tomosyn antibody (ab45228) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 128 kDa
Additional bands at: 30 kDa, 52 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
