Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free (ab232559)
![Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free (ab232559)](https://www.abcam.com/ps/products/232/ab232559/Images/ab232559-393696-anti-calpain-2-antibody-epr5977-western-blot-human-knockout-lysate.jpg "Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free (ab232559)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5977] to Calpain 2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free
See all Calpain 2 primary antibodies -
Description
Rabbit monoclonal [EPR5977] to Calpain 2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat and mouse brain and kidney tissues; HEK293T, A431, LNCaP and PC-3 cell lysates.
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General notes
Ab232559 is the carrier-free version of ab126600. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232559 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5977 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free (ab232559)All lanes : Anti-Calpain 2 antibody [EPR5977] (ab126600) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CAPN2 knockout HEK293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : LNCaP cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab126600).
Lanes 1-4: Merged signal (red and green). Green - ab126600 observed at 80 kDa. Red - loading control ab8245 observed at 36 kDa.
ab126600 Anti-Calpain 2 antibody [EPR5977] was shown to specifically react with Calpain 2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266628 (knockout cell lysate ab257379) was used. Wild-type and Calpain 2 knockout samples were subjected to SDS-PAGE. ab126600 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free (ab232559)](https://www.abcam.com/ps/products/232/ab232559/Images/ab232559-309019-122.jpg)
Western blot - Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free (ab232559)All lanes : Anti-Calpain 2 antibody [EPR5977] (ab126600) at 1/1000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat kidney tissue lysate
Lane 3 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 80 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126600).
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![Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free (ab232559)](https://www.abcam.com/ps/products/232/ab232559/Images/ab232559-3-benefits-of-recombinant-antibodies.png)
Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free (ab232559)
