Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20257] to Myeloperoxidase - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free
    See all Myeloperoxidase primary antibodies

  • Description

    Rabbit monoclonal [EPR20257] to Myeloperoxidase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab221847 is the carrier-free version of ab208670. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab221847 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on neutrophils of human spleen is observed [PMID: 19566938].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

  • Flow Cytometry - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
    Flow Cytometry - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
    Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa cells (left panel) and HL-60 cells (right panel) labeling Myeloperoxidase with ab208670 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control ((ab172730); black) and unlabelled control (cells without incubation with primary and secondary antibodies; blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
     
    Negative control: HeLa (PMID: 12040446).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on neutrophils of human stomach cancer is observed [PMID: 19566938].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on neutrophils of mouse spleen is observed [PMID: 19566938].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on neutrophils of rat spleen is observed [PMID: 19566938].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

  • Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
    Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

    Immunofluorescent analysis of 100% methanol-fixed HL-60 (Human promyelocytic leukemia cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Myeloperoxidase with ab208670 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on HL-60 cell line.

    Negative control: HeLa (PMID: 12040446).

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

  • Flow Cytometry - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
    Flow Cytometry - Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
    Flow cytometric analysis of 4% paraformaldehyde-fixed Mouse PBMC cells labeling Myeloperoxidase with ab208670 at 1/500 dilution (right), compared with a rabbit monoclonal IgG isotype control (ab172730; left). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
     
    Mouse peripheral blood mononuclear cells stained intracellularly with ab208670 (Right) and isotype control (Left). Only monocytes and granulocytes (larger SSC population) result in positive signal while the lymphocyte population remains unchanged.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208670).

  • Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)
    Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Myeloperoxidase antibody [EPR20257] - BSA and Azide free (ab221847)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.