Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4792] to Myeloperoxidase
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Myeloperoxidase antibody [EPR4792]
    See all Myeloperoxidase primary antibodies

  • Description

    Rabbit monoclonal [EPR4792] to Myeloperoxidase

  • Host species

    Rabbit

  • Specificity

    ab109116 recognizes Myeloperoxidase (85-90 kDa) and its light chain (15 kDa).

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HL-60 cell lysate IHC-P: Human colon, lung and spleen tissues

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
    Western blot - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
    Anti-Myeloperoxidase antibody [EPR4792] (ab109116) at 1/1000 dilution + HL-60 cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 84 kDa
    Observed band size: 85-90 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 15 kDa (possible cleavage fragment)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

    ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

    ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human lung tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

    ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human spleen tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

    ab109116 showing positive staining in Normal liver tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

    ab109116 showing positive staining in Bone marrow tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

    ab109116 showing negative staining in Skeletal muscle tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
    Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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