Anti-F-actin antibody [NH3] (ab205)
Key features and details
- Mouse monoclonal [NH3] to F-actin
- Reacts with: Rat, Rabbit, Human
- Isotype: IgM
Overview
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Product name
Anti-F-actin antibody [NH3]
See all F-actin primary antibodies -
Description
Mouse monoclonal [NH3] to F-actin -
Host species
Mouse -
Specificity
Clone NH3 recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary. -
Species reactivity
Reacts with: Rat, Rabbit, Human -
Immunogen
Human monocytes and U397 cell line.
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Epitope
This antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule. -
Positive control
- gastrointestinal tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Purified IgM -
Purification notes
Purified IgM prepared from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
NH3 -
Myeloma
NS1 -
Isotype
IgM -
Light chain type
unknown -
Research areas
