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Neuroscience Cell Type Marker Neuron marker Dendrite marker

Anti-FMRP antibody - BSA and Azide free (Detector) (ab242905)

Anti-FMRP antibody - BSA and Azide free (Detector) (ab242905)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal to FMRP - BSA and Azide free (Detector)
  • Suitable for: WB, Sandwich ELISA
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-FMRP antibody - BSA and Azide free (Detector)
    See all FMRP primary antibodies
  • Description

    Rabbit monoclonal to FMRP - BSA and Azide free (Detector)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Positive control

    • WB: C2C12 cell lysate. Human brain lysate.
  • General notes

    ab242905 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human FMRP. The recommended pair for sandwich ELISA is:
         Capture: ab242660, Human FMRP Capture Antibody (unconjugated)
         Detector: ab242905, Human FMRP Detector Antibody (unconjugated)
    The reference range value is 40 - 2560 pg/ml.

    The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituent: 100% PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Dendrite marker
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Epigenetics and Nuclear Signaling
    • RNAi
    • RISC

Images

  • Western blot - Anti-FMRP antibody - BSA and Azide free (Detector) (ab242905)
    Western blot - Anti-FMRP antibody - BSA and Azide free (Detector) (ab242905)
    All lanes : Anti-FMRP antibody - BSA and Azide free (Detector) (ab242905) at 1/1000 dilution

    Lane 1 : C2C12 (Mouse myoblast cell line) cell lysate
    Lane 2 : Human brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 71 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 37 seconds


    Blocking buffer: 5% NFDM/TBST

  • Anti-FMRP antibody - BSA and Azide free (Detector) (ab242905)
    Anti-FMRP antibody - BSA and Azide free (Detector) (ab242905)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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