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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases

Anti-ErbB4 / HER4 (phospho Y1258) antibody [EP2272AY] (ab76132)

Price and availability

221 126 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-ErbB4 / HER4 (phospho Y1258) antibody [EP2272AY] (ab76132)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP2272AY] to ErbB4 / HER4 (phospho Y1258)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ErbB4 / HER4 (phospho Y1258) antibody [EP2272AY]
    See all ErbB4 / HER4 primary antibodies
  • Description

    Rabbit monoclonal [EP2272AY] to ErbB4 / HER4 (phospho Y1258)
  • Host species

    Rabbit
  • Specificity

    ab76132 detects ErbB4 / HER4 phosphorylated on tyrosine 1258.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    corresponding to Human ErbB4/ HER4. A phospho specific peptide corresponding to residues surrounding tyrosine 1258 of human ErbB4/ HER4

  • Positive control

    • SKBR3 cell lysates treated with EGF.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP2272AY
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Cancer
    • Growth factors
    • EGF
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factors
    • Cancer
    • Tumor biomarkers
    • Oncoproteins

Images

  • Western blot - Anti-ErbB4 / HER4 (phospho Y1258) antibody [EP2272AY] (ab76132)
    Western blot - Anti-ErbB4 / HER4 (phospho Y1258) antibody [EP2272AY] (ab76132)
    All lanes : Anti-ErbB4 / HER4 (phospho Y1258) antibody [EP2272AY] (ab76132) at 1/2000 dilution

    Lane 1 : SKBR3 cell lysates treated with EGF
    Lane 2 : SKBR3 cell lysates untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 147 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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