Anti-ErbB4 / HER4 (phospho Y1258) antibody [EP2272AY] (ab76132)
Key features and details
- Rabbit monoclonal [EP2272AY] to ErbB4 / HER4 (phospho Y1258)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ErbB4 / HER4 (phospho Y1258) antibody [EP2272AY]
See all ErbB4 / HER4 primary antibodies -
Description
Rabbit monoclonal [EP2272AY] to ErbB4 / HER4 (phospho Y1258) -
Host species
Rabbit -
Specificity
ab76132 detects ErbB4 / HER4 phosphorylated on tyrosine 1258.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
corresponding to Human ErbB4/ HER4. A phospho specific peptide corresponding to residues surrounding tyrosine 1258 of human ErbB4/ HER4
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Positive control
- SKBR3 cell lysates treated with EGF.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2272AY -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ErbB4 / HER4 (phospho Y1258) antibody [EP2272AY] (ab76132) at 1/2000 dilution
Lane 1 : SKBR3 cell lysates treated with EGF
Lane 2 : SKBR3 cell lysates untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 147 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?