Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300)
![Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300)](https://www.abcam.com/ps/products/76/ab76300/Images/ab76300-91682-anti-egfr-phospho-s1046-s1047-antibody-ep2259y-western-blot.gif "Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2259Y] to EGFR (phospho S1046 + S1047)
- Suitable for: WB, IP, ICC/IF, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y]
See all EGFR primary antibodies -
Description
Rabbit monoclonal [EP2259Y] to EGFR (phospho S1046 + S1047) -
Host species
Rabbit -
Specificity
Recognises EGFR phosphorylated on Serine 1046 and Serine 1047 of the mature human isoform 1 (corresponding to S1070 and S1071 from the precursor form P00533-1/p170) -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 cell lysates treated with EGF. ICC/IF: A431 cells treated with EGF. IP: A431 treated with EGF whole cell lysate. Dot Blot: EGFR (pS1046/pS1047) phospho peptide.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2259Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300)All lanes : Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300) at 1/1000000 dilution
Lane 1 : A431 cell lysate
Lane 2 : A431 cell lysate treated with EGF
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 134 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300)](https://www.abcam.com/ps/products/76/ab76300/Images/ab76300-285986-anti-egfr-phospho-s1046-s1047-antibody-ep2259y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300)Immunocytochemistry/Immunofluorescence analysis of untreated and EGF (100ng/mL for 10 minutes) treated A431 cells labelling EGFR (phospho S1046 + S1047) with ab76300 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, an Alexa Fluor® 594-conjugated mouse anti-tubulin (1/200). Nuclei counterstained with DAPI (blue).
Control 1: untreated A431 cells incubated with PBS instead of primary antibody followed by incubation with ab150077 Alexa Fluor® 488 goat anti-rabbit IgG.
Control 2: EGF treated A431 cells incubated with PBS instead of primary antibody followed by incubation with ab150077 Alexa Fluor® 488 goat anti-rabbit IgG. -
![Immunoprecipitation - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300)](https://www.abcam.com/ps/products/76/ab76300/Images/ab76300-6-anti-egfr-phospho-s1046-s1047-antibody-ep2259y-immunoprecipitation-a431-human.jpg)
Immunoprecipitation - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300)Purified ab76300 at 1/40 dilution (2µg) immunoprecipitating EGFR in A431 treated with EGF whole cell lysate.
Lane 1 (input): A431 (Human epidermoid carcinoma epithelial cell) treated with EGF whole cell lysate 10µg
Lane 2 (+): ab76300 + A431 treated with EGF whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76300 in A431 treated with EGF whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 180 kDa -
![Dot Blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300)](https://www.abcam.com/ps/products/76/ab76300/Images/ab76300-256545-anti-egfr-phospho-s1046-s1047-antibody-ep2259y-dot-blot.jpg)
Dot Blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300)Primary antibody: ab76300 at a dilution of 1/1000.
Secondary antibody: Peroxidase conjugated-goat anti-rabbit IgG, (H+L) at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST
Lane 1: EGFR (pS1046/pS1047) phospho peptide.
Lane 2: EGFR non-phospho peptide.Exposure time: 3 minutes.
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![Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300)](https://www.abcam.com/ps/products/76/ab76300/Images/ab76300-5-benefits-of-recombinant-antibodies.png)
Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (ab76300)
