Anti-Lamin A + Lamin C antibody [4C4] (ab190380)
Key features and details
- Mouse monoclonal [4C4] to Lamin A + Lamin C
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Lamin A + Lamin C antibody [4C4]
See all Lamin A + Lamin C primary antibodies -
Description
Mouse monoclonal [4C4] to Lamin A + Lamin C -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
Recombinant full length protein corresponding to Human Lamin A + Lamin C aa 1 to the C-terminus. purified form E. coli.
Database link: P02545 -
Positive control
- WB: HAP1, HepG2, and HeLa cell lysates. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4C4 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-Lamin A + Lamin C antibody [4C4] (ab190380) at 1/2000 dilution
Lane 1 : Wild-type HAP1 cell lysate at 40 µg
Lane 2 : LMNA knockout HAP1 cell lysate at 40 µg
Lane 3 : HeLa cell lysate at 20 µg
Lane 4 : HepG2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 64, 74 kDa
Observed band size: 70-75 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab190380 observed at 70-75 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab190380 was shown to react with LMNA in wild-type HAP1 cells in Western blot. Loss of signal was observed when LMNA knockout sample was used. HAP1 wild-type and LMNA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab190380 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Lamin A + C (red) using ab190380 at 1/2000 dilution in ICC/IF. HSP60 is detected with an anti-HSP60 antibody (green). The nuclear counter stain is DAPI (blue).
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All lanes : Anti-Lamin A + Lamin C antibody [4C4] (ab190380) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : C6 whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Predicted band size: 64, 74 kDa
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Immunofluorescent analysis of HeLa cells labeling Lamin A + C with ab190380 at 1/1000 dilution (red) and a chicken polyclonal antibody to Vimentin (green) and DNA (blue).
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Anti-Lamin A + Lamin C antibody [4C4] (ab190380) at 1/10000 dilution + crude HeLa cell extract
Predicted band size: 64, 74 kDa