Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

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Anti-Lamin A + Lamin C antibody [4C4] (ab190380)

Key features and details

Mouse monoclonal [4C4] to Lamin A + Lamin C
Suitable for: ICC/IF, WB
Knockout validated
Reacts with: Mouse, Rat, Human
Isotype: IgG1

Product name

Anti-Lamin A + Lamin C antibody [4C4]
See all Lamin A + Lamin C primary antibodies
Description

Mouse monoclonal [4C4] to Lamin A + Lamin C
Host species

Mouse
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Recombinant full length protein corresponding to Human Lamin A + Lamin C aa 1 to the C-terminus. purified form E. coli.
Database link: P02545

Run BLAST with Run BLAST with
Positive control
- WB: HAP1, HepG2, and HeLa cell lysates. ICC/IF: HeLa cells.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

4C4
Isotype

IgG1
Research areas

Western blot - Anti-Lamin A + Lamin C antibody [4C4] (ab190380)

All lanes : Anti-Lamin A + Lamin C antibody [4C4] (ab190380) at 1/2000 dilution

Lane 1 : Wild-type HAP1 cell lysate at 40 µg
Lane 2 : LMNA knockout HAP1 cell lysate at 40 µg
Lane 3 : HeLa cell lysate at 20 µg
Lane 4 : HepG2 cell lysate at 20 µg

Performed under reducing conditions.

Predicted band size: 64, 74 kDa
Observed band size: 70-75 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab190380 observed at 70-75 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.

ab190380 was shown to react with LMNA in wild-type HAP1 cells in Western blot. Loss of signal was observed when LMNA knockout sample was used. HAP1 wild-type and LMNA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween^®) before incubation with ab190380 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [4C4] (ab190380)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Lamin A + C (red) using ab190380 at 1/2000 dilution in ICC/IF. HSP60 is detected with an anti-HSP60 antibody (green). The nuclear counter stain is DAPI (blue).
Western blot - Anti-Lamin A + Lamin C antibody [4C4] (ab190380)

All lanes : Anti-Lamin A + Lamin C antibody [4C4] (ab190380) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : C6 whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Predicted band size: 64, 74 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [4C4] (ab190380)

Immunofluorescent analysis of HeLa cells labeling Lamin A + C with ab190380 at 1/1000 dilution (red) and a chicken polyclonal antibody to Vimentin (green) and DNA (blue).
Western blot - Anti-Lamin A + Lamin C antibody [4C4] (ab190380)

Anti-Lamin A + Lamin C antibody [4C4] (ab190380) at 1/10000 dilution + crude HeLa cell extract

Predicted band size: 64, 74 kDa

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