Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COX IV antibody [20E8C12] (ab14744)

Key features and details

  • Mouse monoclonal [20E8C12] to COX IV
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-COX IV antibody [20E8C12]
    See all COX IV primary antibodies

  • Description

    Mouse monoclonal [20E8C12] to COX IV

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Cow COX IV.

  • Positive control

    • Human, bovine, murine and rat heart mitochondria.

  • General notes

    This antibody clone is manufactured by Abcam.

    This antibody makes an effective loading control for mitochondria. COXIV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COXIV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Rabbit polyclonal to COX IV antibody (ab16056).

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Western blot - Anti-COX IV antibody [20E8C12] (ab14744)
    Western blot - Anti-COX IV antibody [20E8C12] (ab14744)
    All lanes : Anti-COX IV antibody [20E8C12] (ab14744) at 1/5000 dilution

    Lane 1 : Cytoplasmic fraction mouse NIH/3T3 cell lysate
    Lane 2 : Nuclear fraction mouse NIH/3T3 cell lysate

    Secondary
    All lanes : HRP conjugated goat anti-mouse antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 16 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This image is courtesy of an Abreview submitted by Camilla Skjerpen on 4 July 2005.

    See Abreview

  • Flow Cytometry - Anti-COX IV antibody [20E8C12] (ab14744)
    Flow Cytometry - Anti-COX IV antibody [20E8C12] (ab14744)
    Overlay histogram showing HeLa cells stained with ab14744 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14744, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-COX IV antibody [20E8C12] (ab14744)
    Western blot - Anti-COX IV antibody [20E8C12] (ab14744)
    All lanes : Anti-COX IV antibody [20E8C12] (ab14744) at 1 µg/ml

    Lane 1 : Isolated mitochondria from human heart at 5 µg
    Lane 2 : Isolated mitochondria from bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from rat heart at 10 µg
    Lane 4 : Isolated mitochondria from murine heart at 10 µg

    Observed band size: 16 kDa why is the actual band size different from the predicted?

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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