Alexa Fluor® 405 Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab210180)
![Alexa Fluor® 405 Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab210180)](https://www.abcam.com/ps/products/210/ab210180/Images/ab210180-259646-anti-cox-iv-antibody-epr9442abc-mitochondrial-loading-control-alexa-fluor-405-flow-cytometry.jpg "Alexa Fluor® 405 Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab210180)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 405 Rabbit monoclonal [EPR9442(ABC)] to COX IV - Mitochondrial Loading Control
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
Overview
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Product name
Alexa Fluor® 405 Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control
See all COX IV primary antibodies -
Description
Alexa Fluor® 405 Rabbit monoclonal [EPR9442(ABC)] to COX IV - Mitochondrial Loading Control -
Host species
Rabbit -
Conjugation
Alexa Fluor® 405. Ex: 402nm, Em: 421nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9442(ABC) -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Alexa Fluor® 405 Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab210180)
Overlay histogram showing HeLa cells stained with ab210180 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22ºC and then blocked with 1x PBS / 10% normal goat serum for 1 hr at 22ºC. Cells were then incubated with the antibody (ab210180, 1/500 dilution) for 30 min at 22ºC.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW violet laser (405nm) and 450/50 bandpass filter.
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![Alexa Fluor® 405 Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab210180)](https://www.abcam.com/ps/products/210/ab210180/Images/ab210180-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 405 Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab210180)
