Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9442(ABC)] to COX IV - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free
    See all COX IV primary antibodies

  • Description

    Rabbit monoclonal [EPR9442(ABC)] to COX IV - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal heart lysate; HepG2 whole cell lysate; Mouse and rat heart lysates. IHC-P: Human hepatocellular carcinoma, Human cervix carcinoma, mouse kidney and rat cardiac muscle tissues. ICC/IF: HeLa and HepG2 cells. Flow Cyt: MCF7 cells. IP: Human fetal heart whole cell lysate.

  • General notes

    Ab231168 is the carrier-free version of ab202554. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab231168 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)
    Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling COX IV with ab202554 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Cytoplasmic staining on HepG2 cells is observed.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202554 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202554).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasmic staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202554).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasmic staining on mouse kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202554).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasmic staining on Human cervix carcinoma tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202554).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)
    Flow Cytometry - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)

    Flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling COX IV with ab202554 at 1/20 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202554).

  • Immunoprecipitation - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)
    Immunoprecipitation - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)

    COX IV was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab202554 at 1/20 dilution.

    Western blot was performed from the immunoprecipitate using ab202554 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

    Lane 1: Human fetal heart whole cell lysate 10 µg (Input).

    Lane 2: ab202554 IP in Human fetal heart whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202554 in Human fetal heart whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202554).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)

    This IHC data was generated using the same anti-COX IV antibody clone, EPR9442(ABC), in a different buffer formulation (cat# ab202554).

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasmic staining on Human hepatocellular carcinoma tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)
    Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)

    This ICC data was generated using the same anti-COX IV antibody clone, EPR9442(ABC), in a different buffer formulation (cat# ab202554).

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling COX IV with ab202554 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Cytoplasmic staining on HeLa cells is observed.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202554 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)
    Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)

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