Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AMPK gamma 1 antibody [Y307] - BSA and Azide free (ab247251)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y307] to AMPK gamma 1 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-AMPK gamma 1 antibody [Y307] - BSA and Azide free
    See all AMPK gamma 1 primary antibodies

  • Description

    Rabbit monoclonal [Y307] to AMPK gamma 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    ab247251 reacts with an epitope located in the N terminal region of AMPK gamma 1.

  • General notes

    ab247251 is the carrier-free version of ab32382 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247251 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-AMPK gamma 1 antibody [Y307] - BSA and Azide free (ab247251)
    Western blot - Anti-AMPK gamma 1 antibody [Y307] - BSA and Azide free (ab247251)
    All lanes : Anti-AMPK gamma 1 antibody [Y307] (ab32382) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : PRKAG1 (AMPK gamma 1) knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 38 kDa



    This data was developed using ab32382, the same antibody clone in a different buffer formulation.

    Lanes 1 - 3: Merged signal (red and green). Green - ab32382 observed at 38 kDa. Red - loading control, Mouse anti-Vinculin, observed at 125 kDa.

    ab32382 was shown to recognize AMPK gamma 1 in wild-type HAP1 cells as signal was lost at the expected MW in PRKAG1 (AMPK gamma 1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PRKAG1 (AMPK gamma 1) knockout samples were subjected to SDS-PAGE. ab32382 and Mouse anti-Vinculin (ab9484 loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-AMPK gamma 1 antibody [Y307] - BSA and Azide free (ab247251)
    Western blot - Anti-AMPK gamma 1 antibody [Y307] - BSA and Azide free (ab247251)
    All lanes : Anti-AMPK gamma 1 antibody [Y307] (ab32382) at 1/2000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate

    Predicted band size: 38 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32382, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK gamma 1 antibody [Y307] - BSA and Azide free (ab247251)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK gamma 1 antibody [Y307] - BSA and Azide free (ab247251)
    This data was developed using ab32382, the same antibody clone in a different buffer formulation.This image shows paraffin embedded human skeletal muscle stained with 1/250 ab23282. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-AMPK gamma 1 antibody [Y307] - BSA and Azide free (ab247251)
    Anti-AMPK gamma 1 antibody [Y307] - BSA and Azide free (ab247251)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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