Anti-Niemann Pick C2 antibody [EPR19993] - BSA and Azide free (ab271975)
![Anti-Niemann Pick C2 antibody [EPR19993] - BSA and Azide free (ab271975)](https://www.abcam.com/ps/products/271/ab271975/Images/ab271975-4-ab207158265994antiniemannpickc2antibodyepr19993immunohistochemistry.jpg "Anti-Niemann Pick C2 antibody [EPR19993] - BSA and Azide free (ab271975)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19993] to Niemann Pick C2 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Niemann Pick C2 antibody [EPR19993] - BSA and Azide free
See all Niemann Pick C2 primary antibodies -
Description
Rabbit monoclonal [EPR19993] to Niemann Pick C2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal kidney and spleen lysates; HepG2, C6, RAW 264,7, PC-12 and NIH/3T3 whole cell lysates; mouse spleen lysate; rat kidney lysate.IHC-P: Human endometrium and endometrium cancer tissues; mouse testis tissue; rat kidney tissue.
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General notes
ab271975 is the carrier-free version of ab207158. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19993 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - BSA and Azide free (ab271975)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Niemann Pick C2 with ab207158 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat kidney is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207158). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - BSA and Azide free (ab271975)](https://www.abcam.com/ps/products/271/ab271975/Images/ab271975-3-ab207158265997antiniemannpickc2antibodyepr19993immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - BSA and Azide free (ab271975)Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling Niemann Pick C2 with ab207158 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human endometrium is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207158). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - BSA and Azide free (ab271975)](https://www.abcam.com/ps/products/271/ab271975/Images/ab271975-2-ab207158265996antiniemannpickc2antibodyepr19993immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - BSA and Azide free (ab271975)Immunohistochemical analysis of paraffin-embedded human endometrium cancer tissue labeling Niemann Pick C2 with ab207158 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human endometrium cancer is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207158). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - BSA and Azide free (ab271975)](https://www.abcam.com/ps/products/271/ab271975/Images/ab271975-1-ab207158265995antiniemannpickc2antibodyepr19993immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody [EPR19993] - BSA and Azide free (ab271975)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Niemann Pick C2 with ab207158 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse testis is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207158). -
![Anti-Niemann Pick C2 antibody [EPR19993] - BSA and Azide free (ab271975)](https://www.abcam.com/ps/products/271/ab271975/Images/ab271975-5-benefits-of-recombinant-antibodies.png)
Anti-Niemann Pick C2 antibody [EPR19993] - BSA and Azide free (ab271975)
