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Anti-ATP5O antibody [4C11C10D12] (ab110276)

Key features and details

  • Mouse monoclonal [4C11C10D12] to ATP5O
  • Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
  • Reacts with: Mouse, Cow, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-ATP5O antibody [4C11C10D12]
    See all ATP5O primary antibodies

  • Description

    Mouse monoclonal [4C11C10D12] to ATP5O

  • Host species

    Mouse

  • Specificity

    Antigen retrieval is required for ICC. Immediately before the permeabilization step, heat the coverslips for 10 minutes in 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Cow
    Human
    African green monkey
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • Isolated mitochondria from Human heart and Bovine heart, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), Human colon tissue, HL-60 cells

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
    Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
    All lanes : Anti-ATP5O antibody [4C11C10D12] (ab110276) at 0.5 µg/ml

    Lane 1 : Human heart mitochondria at 5 µg
    Lane 2 : Bovine heart mitochondria at 1 µg

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody [4C11C10D12] (ab110276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody [4C11C10D12] (ab110276)
    Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5O antibody [4C11C10D12] (ab110276)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP5O antibody [4C11C10D12] (ab110276)
    Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
  • Flow Cytometry - Anti-ATP5O antibody [4C11C10D12] (ab110276)
    Flow Cytometry - Anti-ATP5O antibody [4C11C10D12] (ab110276)
    HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
    Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
    All lanes : Anti-ATP5O antibody [4C11C10D12] (ab110276) at 1 µg/ml

    Lane 1 : HDFn (human) cell lysates at 20 µg
    Lane 2 : COS7 (monkey) cell lysates at 20 µg
    Lane 3 : H4IIE (rat) cell lysates at 20 µg
    Lane 4 : MEF (mouse) cell lysates at 20 µg
    Lane 5 : bovine heart mitochondria lysates at 5 µg

    Predicted band size: 23 kDa



    Secondary antibody: Goat-Anti-Mouse-IR800 1:4000

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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