Human Apolipoprotein B ELISA Kit (ab277393)
Key features and details
- Sensitivity: 2.5 ng/ml
- Range: 2.458 ng/ml - 600 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Apolipoprotein B ELISA Kit
See all Apolipoprotein B kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.5 ng/ml -
Range
2.458 ng/ml - 600 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 103.4 94% - 120% Plasma 103.4 87% - 114% Cell culture media 101 73% - 127% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Apolipoprotein B ELISA Kit (ab277393) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Apolipoprotein B in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Apolipoprotein B coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Apolipoprotein B present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Apolipoprotein B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Apolipoprotein B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Human ApolipoproteinB Antibody 2 vials Anti-Human Apolipoprotein B coated Microplate 1 unit Human Apolipoprotein B standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Function
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. -
Involvement in disease
Hypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. -
Sequence similarities
Contains 1 vitellogenin domain. -
Post-translational
modificationsPalmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
-
Alternative names
- Apo B
- Apo B 100
- Apo B-100
see all -
Database links
- Entrez Gene: 338 Human
- Omim: 107730 Human
- SwissProt: P04114 Human
- Unigene: 120759 Human
Images
-
Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.