Anti-Histone H1 antibody (ab61177)
Key features and details
- Rabbit polyclonal to Histone H1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H1 antibody
See all Histone H1 primary antibodies -
Description
Rabbit polyclonal to Histone H1 -
Host species
Rabbit -
Specificity
Detects total Histone H1 levels. -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H1 aa 15-19. A non-phosphopeptide derived from human Histone H1 around the phosphorylation site of threonine 17
Sequence:E-K-T-P-V
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H1 antibody (ab61177)All lanes : Anti-Histone H1 antibody (ab61177)
Lane 1 : HepG2
Lane 2 : HepG2 with blocking peptide
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody (ab61177)IHC image of ab61177 staining Histone H1 in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab61177, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 antibody (ab61177)ab61177 at 1/500 - 1/1000 dilution staining Histone H1 in HuvEc cells, treated with serum (20%, 15mins) by Immunofluorescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1 antibody (ab61177)Paraffin-embedded human colon carcinoma tissue stained for Histone H1 with ab61177 at 1/50 dilution in immunohistochemical analysis.
The sample in the right-hand panel is incubated with the immunizing peptide.
