Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HCN2 antibody [4A6] (ab252836)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [4A6] to HCN2
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HCN2 antibody [4A6]
    See all HCN2 primary antibodies

  • Description

    Rat monoclonal [4A6] to HCN2

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Human, mouse and rat cerebrum tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling HCN2 with ab252836 at 1.235µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat cerebrum tissue is observed. The section was incubated with ab252836 for 30mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling HCN2 with ab252836 at 1.235µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse cerebrum tissue is observed. The section was incubated with ab252836 for 30mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling HCN2 with ab252836 at 1.235µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human cerebrum tissue is observed. The section was incubated with ab252836 for 30mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Anti-HCN2 antibody [4A6] (ab252836)
    Anti-HCN2 antibody [4A6] (ab252836)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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