Vector contains kanamycin resistance for plant selection, chloramphenicol resistance for bacterial selection and no reporter gene.

The pCambia220 vector offers:
High copy number in E.coli for high DNA yields
pVS1 replicon for high stability in Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with chloramphenicol
Plant selection with kanamycin

Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV₃₅S promoter and terminated by the CaMV₃₅S polyA signal.

This vector contains minimal heterologous sequences for plant transformation and selection of transformants, allowing insertion of desired genes for transformation into plants but require all promoter and terminator sequences for plant expression of newly cloned genes. The pUC18 polylinker within the lacZa fragment allows blue/white screening of clones in E. coli cloning work.

Genbank Sequence data

Concentration of Antibiotics for selection.
In bacterial system:
Chloramphenicol resistance (cat – chloramphenicol acetyltransferase from E.coli Tn-cam204) in bacteria: use 10μg/mL in E.coli and A.tumefaciens.

In plant system: