Conjugation Check Kit (ab236554)
Key features and details
- Assay time: 10 min
Overview
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Product name
Conjugation Check Kit -
Assay time
0h 10m -
Product overview
Conjugation Check Kit (ab236554) confirms the conjugation of an antibody in one easy step.
The key component of the kit is a nitrocellulose membrane containing a ‘Test line’ of immobilized Protein A and Protein G called a “half strip”.
Both Protein A and Protein G have a high affinity for the Fc region of a variety of IgG molecules. The “half strips” also contain an absorbent pad to promote and control the flow of sample through the nitrocellulose. This simple qualitative lateral flow assay does not require any specialized or costly equipment.
When the antibody conjugate is run on the Protein A/G strip, it flows along the nitrocellulose, binding to the Protein A and Protein G concentrated at the Test line. When the antibody is successfully conjugated to a colored label, a visible line appears on the strip.
*Please note Conjugate Check Kit is not compatible with the following AMCA, DyLight® 350, Atto390, DyLight® 405, DyLight® 800, DyLight® 755, Alex Fluor® 700 and DyLight® 680.
Benefits:
Quick and easy qualitative test to check success of conjugation
Compatible with antibody conjugates generated using our Lightning-Link® Fluorescent Labelling kits, Gold and Latex Conjugation Kits
Compatible with any other conjugation technology that uses colored labels
No costly equipment required
Small sample volumes required (40 μL)
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Notes
This product is manufactured by Expedeon, an Abcam company, and was previously called Conjugate Check&Go! Antibody Labeling Confirmation Kit. 4000-0030 is the same as the 30-tests size.
Properties
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Storage instructions
Please refer to protocols. -
Components 30 tests ab274083 - Conjugation Checking Kit Positive Control 1 vial ab274081 - Protein A/G Strips 1 x 30 tests ab274082 - Running Buffer (10X) 2 x 1500µl
Images
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Schematic representation of Conjugation Check Kit process
When the antibody conjugate is run on the Protein A/G strip, it flows along the nitrocellulose, binding to the Protein A and Protein G concentrated on the Test line. When the antibody is successfully conjugated to a colored label, a visible line appears on the strip.