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Anti-GCSF Receptor antibody [8F8B12] (ab233783)

Price and availability

261 331 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-GCSF Receptor antibody [8F8B12] (ab233783)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [8F8B12] to GCSF Receptor
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-GCSF Receptor antibody [8F8B12]
    See all GCSF Receptor primary antibodies
  • Description

    Mouse monoclonal [8F8B12] to GCSF Receptor
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human GCSF Receptor aa 1-200. (Expressed in E.coli).
    Database link: Q99062

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Recombinant human GCSF Receptor (AA: extra 25-187) protein; A431, K562, THP-1, MOLT-4, Jurkat and GCSF Receptor (AA: extra 25-187)-hIgGFc transfected HEK-293 whole cell lysate. IHC-P: Human bladder cancer tissue. Flow: Ramos cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    8F8B12
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • CSFs
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors

Images

  • Flow Cytometry - Anti-GCSF Receptor antibody [8F8B12] (ab233783)
    Flow Cytometry - Anti-GCSF Receptor antibody [8F8B12] (ab233783)

    Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling GCSF Receptor with ab233783 at 1/200 dilution (green) compared with a negative control (red).

  • Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783)
    Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783)
    Anti-GCSF Receptor antibody [8F8B12] (ab233783) at 1/500 dilution + Recombinant human GCSF receptor (AA: extra 25-187) protein

    Developed using the ECL technique.


    Expected MW is 46.5 kDa.

  • Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783)
    Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783)
    All lanes : Anti-GCSF Receptor antibody [8F8B12] (ab233783) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : GCSF Receptor (AA: extra 25-187)-hIgGFc transfected HEK-293 whole cell lysate

    Developed using the ECL technique.
  • Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783)
    Western blot - Anti-GCSF Receptor antibody [8F8B12] (ab233783)
    All lanes : Anti-GCSF Receptor antibody [8F8B12] (ab233783) at 1/500 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 4 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
    Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Developed using the ECL technique.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCSF Receptor antibody [8F8B12] (ab233783)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCSF Receptor antibody [8F8B12] (ab233783)

    Paraffin-embedded human bladder cancer tissue stained for GCSF Receptor using ab233783 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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