Anti-GCSF Receptor antibody [8F8B12] (ab233783)
Key features and details
- Mouse monoclonal [8F8B12] to GCSF Receptor
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-GCSF Receptor antibody [8F8B12]
See all GCSF Receptor primary antibodies -
Description
Mouse monoclonal [8F8B12] to GCSF Receptor -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human GCSF Receptor aa 1-200. (Expressed in E.coli).
Database link: Q99062 -
Positive control
- WB: Recombinant human GCSF Receptor (AA: extra 25-187) protein; A431, K562, THP-1, MOLT-4, Jurkat and GCSF Receptor (AA: extra 25-187)-hIgGFc transfected HEK-293 whole cell lysate. IHC-P: Human bladder cancer tissue. Flow: Ramos cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
8F8B12 -
Isotype
IgG1 -
Research areas
Images
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Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling GCSF Receptor with ab233783 at 1/200 dilution (green) compared with a negative control (red).
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Anti-GCSF Receptor antibody [8F8B12] (ab233783) at 1/500 dilution + Recombinant human GCSF receptor (AA: extra 25-187) protein
Developed using the ECL technique.Expected MW is 46.5 kDa.
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All lanes : Anti-GCSF Receptor antibody [8F8B12] (ab233783) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : GCSF Receptor (AA: extra 25-187)-hIgGFc transfected HEK-293 whole cell lysate
Developed using the ECL technique.
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All lanes : Anti-GCSF Receptor antibody [8F8B12] (ab233783) at 1/500 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 4 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Developed using the ECL technique.
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Paraffin-embedded human bladder cancer tissue stained for GCSF Receptor using ab233783 at 1/200 dilution in immunohistochemical analysis. DAB staining.