ICAD overexpression 293T lysate (whole cell) (ab94130)
Overview
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Product name
ICAD overexpression 293T lysate (whole cell) -
General notes
ab94130 is a 293T cell transfected lysate in which Human ICAD has been transiently over-expressed using a pCMV-ICAD plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Inhibitor of the caspase-activated DNase (DFF40). Similarity: Contains 1 CIDE-N domain. PTM: Caspase-3 cleaves DFF45 at 2 sites to generate an active factor. Phosphorylated upon DNA damage, probably by ATM or ATR.
Images
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SDS-PAGE - ICAD overexpression 293T lysate (whole cell) (ab94130)ab94130 at 15µg/lane on an SDS-PAGE gel.
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Western blot - ICAD overexpression 293T lysate (whole cell) (ab94130)All lanes : Anti-ICAD antibody (ab90563) at 1/500 dilution
Lane 1 :ICAD overexpression 293T lysate (whole cell) (ab94130)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution