Anti-ALDH1L1 antibody [2E7] (ab277623)
![Anti-ALDH1L1 antibody [2E7] (ab277623)](https://www.abcam.com/ps/products/277/ab277623/Images/ab277623-432705-AntiALDH1L1-antibody-2E7-ICC-E20-rat-cortical-neuronglial-cells.JPG "Anti-ALDH1L1 antibody [2E7] (ab277623)")
Key features and details
- Mouse monoclonal [2E7] to ALDH1L1
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
-
Product name
Anti-ALDH1L1 antibody [2E7]
See all ALDH1L1 primary antibodies -
Description
Mouse monoclonal [2E7] to ALDH1L1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human ALDH1L1 aa 402-902.
Database link: O75891 -
Positive control
- WB: Rat liver, kidney, brain, and spinal cord tissue; mouse brain and spinal cord tissues; NIH-3T3, and HEK293 cell lysates. ICC: E20 rat (cortical neuron-glial cells).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from Tissue Culture supernatant. -
Clonality
Monoclonal -
Clone number
2E7 -
Isotype
IgG1 -
Research areas
Images
-
Immunocytochemistry - Anti-ALDH1L1 antibody [2E7] (ab277623)
Cortical neuron-glial cell culture from E20 rat stained for ALDH1L1 (red) using ab277623 at 1/1000 dilution and counter-stained with chicken pAb to GFAP (green) at 1/5000 dilution in Immunocytochemistry. The blue is Hoechst is staining nuclear DNA. ab277623 produces cytoplasmic staining of glial cells, while the GFAP antibody labels the intermediate filament cytoskeleton in astrocytes and other glial cells. Some astrocytic cells express both ALDH1L1 and GFAP and therefore appear yellow.
-
![Western blot - Anti-ALDH1L1 antibody [2E7] (ab277623)](https://www.abcam.com/ps/products/277/ab277623/Images/ab277623-432704-AntiALDH1L1-antibody-2E7-WB-rat-liver-kidney-brain-and-spinal-cord-mouse-brain-and-spinal-cord-NIH3T3-and-HEK293.JPG)
Western blot - Anti-ALDH1L1 antibody [2E7] (ab277623)Lanes 2-11 : Anti-ALDH1L1 antibody [2E7] (ab277623) at 1/5000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : Rat heart tissue
Lane 3 : Rat liver tissue
Lane 4 : Rat kidney tissue
Lane 5 : Rat lung tissue
Lane 6 : Rat brain tissue
Lane 7 : Rat spinal cord tissue
Lane 8 : Mouse brain tissue
Lane 9 : Mouse spinal cord tissue
Lane 10 : NIH/3T3 cell lysate
Lane 11 : HEK-293 cell lysate
Predicted band size: 99 kDa