Anti-CD137 antibody [EPR20238] - BSA and Azide free (ab240382)
![Anti-CD137 antibody [EPR20238] - BSA and Azide free (ab240382)](https://www.abcam.com/ps/products/240/ab240382/Images/ab240382-408318-anti-cd137-antibody-epr20238-bsa-and-azide-free-western-blot.jpg "Anti-CD137 antibody [EPR20238] - BSA and Azide free (ab240382)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20238] to CD137 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-CD137 antibody [EPR20238] - BSA and Azide free
See all CD137 primary antibodies -
Description
Rabbit monoclonal [EPR20238] to CD137 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab240382 is the carrier-free version of ab209256. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240382 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20238 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD137 antibody [EPR20238] - BSA and Azide free (ab240382)Anti-CD137 antibody [EPR20238] (ab209256) at 1/5000 dilution + His-tagged CD137 Fc chimera recombinant protein containing human CD137 (aa24-186) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 4 secondsThis data was developed using ab209256, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-CD137 antibody [EPR20238] - BSA and Azide free (ab240382)](https://www.abcam.com/ps/products/240/ab240382/Images/ab240382-406199-anti-cd137-antibody-epr20238-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CD137 antibody [EPR20238] - BSA and Azide free (ab240382)All lanes : Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution
Lane 1 : Human lymphoma lysate
Lane 2 : Human fetal spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab209256, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The MW observed is consistent with the literature: PMID 10528184; 17560335.
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![Western blot - Anti-CD137 antibody [EPR20238] - BSA and Azide free (ab240382)](https://www.abcam.com/ps/products/240/ab240382/Images/ab240382-403448-anti-cd137-antibody-epr20238-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CD137 antibody [EPR20238] - BSA and Azide free (ab240382)Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution + H9 (Human cutaneous T Lymphocyte cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab209256, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The MW observed is consistent with the literature: PMID 10528184; 17560335.
-
![Western blot - Anti-CD137 antibody [EPR20238] - BSA and Azide free (ab240382)](https://www.abcam.com/ps/products/240/ab240382/Images/ab240382-399289-anti-cd137-antibody-epr20238-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CD137 antibody [EPR20238] - BSA and Azide free (ab240382)All lanes : Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution
Lane 1 : Whole cell lysate from untreated Jurkat (Human T cell leukemia cell line from peripheral blood)
Lane 2 : Whole cell lysate from Jurkat cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (TPA) and 1µM Ionomycin (ab120116) for 48 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 36,40 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab209256, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The expression level of CD137 can be induced by TPA and Ionomycin (PMID: 14648705).
The MW observed is consistent with the literature (PMID: 10528184; 17560335).
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![Anti-CD137 antibody [EPR20238] - BSA and Azide free (ab240382)](https://www.abcam.com/ps/products/240/ab240382/Images/ab240382-5-benefits-of-recombinant-antibodies.png)
Anti-CD137 antibody [EPR20238] - BSA and Azide free (ab240382)
