Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-THEA antibody [EPR18792] - BSA and Azide free (ab250248)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18792] to THEA - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-THEA antibody [EPR18792] - BSA and Azide free
    See all THEA primary antibodies

  • Description

    Rabbit monoclonal [EPR18792] to THEA - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250248 is the carrier-free version of ab180745 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250248 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ACOT11

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-THEA antibody [EPR18792] - BSA and Azide free (ab250248)
    Western blot - Anti-THEA antibody [EPR18792] - BSA and Azide free (ab250248)
    All lanes : Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal heart tissue lysate
    Lane 3 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    This data was developed using ab180745, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lanes 2-3: 15 seconds.

  • Western blot - Anti-THEA antibody [EPR18792] - BSA and Azide free (ab250248)
    Western blot - Anti-THEA antibody [EPR18792] - BSA and Azide free (ab250248)
    All lanes : Anti-THEA antibody [EPR18792] (ab180745) at 1/2000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : LNCaP (human prostate cancer cell line) whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa
    Observed band size: 67,68 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab180745, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lanes 2-3: 15 seconds.

    This antibody can recognize 2 isoforms in human, the predicted MW is 68 & 67 KDa.

  • Western blot - Anti-THEA antibody [EPR18792] - BSA and Azide free (ab250248)
    Western blot - Anti-THEA antibody [EPR18792] - BSA and Azide free (ab250248)
    All lanes : Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution

    Lane 1 : Mouse brown adipose lysate
    Lane 2 : Mouse liver lysate
    Lane 3 : Mouse kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    This data was developed using ab180745, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The expression profile observed is consistent with what has been described in the literature (PMID: 22427358 and 11696000).

  • Western blot - Anti-THEA antibody [EPR18792] - BSA and Azide free (ab250248)
    Western blot - Anti-THEA antibody [EPR18792] - BSA and Azide free (ab250248)
    All lanes : Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    This data was developed using ab180745, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-2: 1 minute; Lane 3: 10 seconds; Lane 4: 15 seconds.

  • Anti-THEA antibody [EPR18792] - BSA and Azide free (ab250248)
    Anti-THEA antibody [EPR18792] - BSA and Azide free (ab250248)

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