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Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)

Key features and details

  • Mouse monoclonal [OTI4G10] to PI 3 Kinase catalytic subunit gamma/PI3K-gamma
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human, African green monkey
  • Isotype: IgG2a

Overview

  • Product name

    Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10]
    See all PI 3 Kinase catalytic subunit gamma/PI3K-gamma primary antibodies

  • Description

    Mouse monoclonal [OTI4G10] to PI 3 Kinase catalytic subunit gamma/PI3K-gamma

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    African green monkey
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human PI 3 Kinase catalytic subunit gamma/PI3K-gamma aa 1-1102. Produced in HEK-293T cells (NP_002640).
    Sequence:

    MELENYKQPVVLREDNCRRRRRMKPRSAAASLSSMELIPIEFVLPTSQRK CKSPETALLHVAGHGNVEQMKAQVWLRALETSVAADFYHRLGPHHFLLLY QKKGQWYEIYDKYQVVQTLDCLRYWKATHRSPGQIHLVQRHPPSEESQAF QRQLTALIGYDVTDVSNVHDDELEFTRRGLVTPRMAEVASRDPKLYAMHP WVTSKPLPEYLWKKIANNCIFIVIHRSTTSQTIKVSPDDTPGAILQSFFT KMAKKKSLMDIPESQSEQDFVLRVCGRDEYLVGETPIKNFQWVRHCLKNG EEIHVVLDTPPDPALDEVRKEEWPLVDDCTGVTGYHEQLTIHGKDHESVF TVSLWDCDRKFRVKIRGIDIPVLPRNTDLTVFVEANIQHGQQVLCQRRTS PKPFTEEVLWNVWLEFSIKIKDLPKGALLNLQIYCGKAPALSSKASAESP SSESKGKVQLLYYVNLLLIDHRFLLRRGEYVLHMWQISGKGEDQGSFNAD KLTSATNPDKENSMSISILLDNYCHPIALPKHQPTPDPEGDRVRAEMPNQ LRKQLEAIIATDPLNPLTAEDKELLWHFRYESLKHPKAYPKLFSSVKWGQ QEIVAKTYQLLARREVWDQSALDVGLTMQLLDCNFSDENVRAIAVQKLES LEDDDVLHYLLQLVQAVKFEPYHDSALARFLLKRGLRNKRIGHFLFWFLR SEIAQSRHYQQRFAVILEAYLRGCGTAMLHDFTQQVQVIEMLQKVTLDIK SLSAEKYDVSSQVISQLKQKLENLQNSQLPESFRVPYDPGLKAGALAIEK CKVMASKKKPLWLEFKCADPTALSNETIGIIFKHGDDLRQDMLILQILRI MESIWETESLDLCLLPYGCISTGDKIGMIEIVKDATTIAKIQQSTVGNTG AFKDEVLNHWLKEKSPTEEKFQAAVERFVYSCAGYCVATFVLGIGDRHND NIMITETGNLFHIDFGHILGNYKSFLGINKERVPFVLTPDFLFVMGTSGK KTSPHFQKFQDICVKAYLALRHHTNLLIILFSMMLMTGMPQLTSKEDIEY IRDALTVGKNEEDAKKYFLDQIEVCRDKGWTVQFNWFLHLVLGIKQGEKH SA


    Database link: P48736
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T cell lysate transfected with pCMV6-ENTRYPI 3 Kinase catalytic subunit gamma/PI3K-gamma cDNA. IHC-P: Human ovary adenocarcinoma and pancreas tissues. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY PI 3 Kinase catalytic subunit gamma/PI3K-gamma cDNA.

  • General notes

    Clone OTI4G10 (formerly 4G10).

    Previously labelled as PI 3 Kinase catalytic subunit gamma. 

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)

    Paraffin-embedded human ovary adenocarcinoma tissue stained for PI 3 Kinase catalytic subunit gamma/PI3K-gamma using ab140307 at 1/150 dilution in immunohistochemical analysis.

  • Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)
    Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)
    All lanes : Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY PI 3 Kinase catalytic subunit gamma/PI3K-gamma cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 126 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)

    COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY PI 3 Kinase catalytic subunit gamma/PI3K-gamma cDNA, labelling PI 3 Kinase catalytic subunit gamma/PI3K-gamma (green) with ab140307 at 1/100 dilution in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)

    Paraffin-embedded human pancreas tissue stained for PI 3 Kinase catalytic subunit gamma/PI3K-gamma using ab140307 at 1/150 dilution in immunohistochemical analysis.

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