Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598)
Key features and details
- Rabbit polyclonal to PI 3 Kinase catalytic subunit gamma/PI3K-gamma
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody
See all PI 3 Kinase catalytic subunit gamma/PI3K-gamma primary antibodies -
Description
Rabbit polyclonal to PI 3 Kinase catalytic subunit gamma/PI3K-gamma -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human PI 3 Kinase catalytic subunit gamma/PI3K-gamma aa 1041-1102 (C terminal).
Database link: P48736 -
Positive control
- WB: Transfected HEK-293T, Neuro 2A, RAW264.7, C8D30 and PC-12 cell lysate; IHC-P: Human breast cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue staining PI 3 Kinase catalytic subunit gamma with ab154598 at 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598)All lanes : Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : PI3K-gamma-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 126 kDa
7.5% SDS PAGE -
Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598)Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598) at 1/1000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Predicted band size: 126 kDa7.5% SDS-PAGE
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Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598)All lanes : Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598) at 1/500 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 126 kDa5% SDS-PAGE
