Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CENPC antibody [EPR15939] - BSA and Azide free (ab232614)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15939] to CENPC - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CENPC antibody [EPR15939] - BSA and Azide free
    See all CENPC primary antibodies

  • Description

    Rabbit monoclonal [EPR15939] to CENPC - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, A431, K562 and NIH/3T3 cell lysates. IHC-P: Human spleen tissue; Rat cardiac muscle tissue. Flow Cyt (intra): K562 cells.

  • General notes

    ab232614 is the carrier-free version of ab193666.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPC antibody [EPR15939] - BSA and Azide free (ab232614)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPC antibody [EPR15939] - BSA and Azide free (ab232614)

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling CENPC with ab193666 at 1/1000 dilution (2μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193666).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-CENPC antibody [EPR15939] - BSA and Azide free (ab232614)
    Flow Cytometry - Anti-CENPC antibody [EPR15939] - BSA and Azide free (ab232614)

    Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling CENPC with ab193666 at 1/400 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193666).

  • Immunoprecipitation - Anti-CENPC antibody [EPR15939] - BSA and Azide free (ab232614)
    Immunoprecipitation - Anti-CENPC antibody [EPR15939] - BSA and Azide free (ab232614)

    Western blot analysis of CENPC immunoprecipitated from Jurkat cell lysate using ab193666 at 1/100 dilution.

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193666).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPC antibody [EPR15939] - BSA and Azide free (ab232614)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPC antibody [EPR15939] - BSA and Azide free (ab232614)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CENPC with ab193666 at 1/1,000 dilution, followed by prediluted HRP polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193666).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-CENPC antibody [EPR15939] - BSA and Azide free (ab232614)
    Anti-CENPC antibody [EPR15939] - BSA and Azide free (ab232614)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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