Anti-MIS12 antibody (ab70843)
Key features and details
- Rabbit polyclonal to MIS12
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MIS12 antibody -
Description
Rabbit polyclonal to MIS12 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan, Bat
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Immunogen
Synthetic peptide corresponding to Human MIS12 (C terminal).
Database link: Q9H081 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-MIS12 antibody (ab70843)HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70843 at 1 µg/ml for WB, subsequent to being used for IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds. -
Western blot - Anti-MIS12 antibody (ab70843)All lanes : Anti-MIS12 antibody (ab70843) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIS12 antibody (ab70843)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling MIS12 with ab70843 at 1/1000 (1µg/ml). Detection: DAB.
