Anti-CENPC antibody [EPR15939] (ab193666)
![Anti-CENPC antibody [EPR15939] (ab193666)](https://www.abcam.com/ps/products/193/ab193666/Images/ab193666-231092-anti-cenpc-antibody-epr15939-immunohistochemistry.jpg "Anti-CENPC antibody [EPR15939] (ab193666)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15939] to CENPC
- Suitable for: IP, Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CENPC antibody [EPR15939]
See all CENPC primary antibodies -
Description
Rabbit monoclonal [EPR15939] to CENPC -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P RatHumanIP HumanWB MouseHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, A431, K562 and NIH/3T3 cell lysates. IHC-P: Human spleen tissue; Rat cardiac muscle tissue. Flow Cyt: K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15939 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPC antibody [EPR15939] (ab193666)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CENPC with ab193666 at 1/1,000 dilution, followed by prediluted HRP polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-CENPC antibody [EPR15939] (ab193666)](https://www.abcam.com/ps/products/193/ab193666/Images/ab193666-231095-anti-cenpc-antibody-epr15939-western-blot.jpg)
Western blot - Anti-CENPC antibody [EPR15939] (ab193666)All lanes : Anti-CENPC antibody [EPR15939] (ab193666) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 107 kDa
Observed band size: 107 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-CENPC antibody [EPR15939] (ab193666)](https://www.abcam.com/ps/products/193/ab193666/Images/ab193666-231094-anti-cenpc-antibody-epr15939-western-blot.jpg)
Western blot - Anti-CENPC antibody [EPR15939] (ab193666)Anti-CENPC antibody [EPR15939] (ab193666) at 1/10000 dilution + K562 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 107 kDa
Observed band size: 107 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-CENPC antibody [EPR15939] (ab193666)](https://www.abcam.com/ps/products/193/ab193666/Images/ab193666-231093-anti-cenpc-antibody-epr15939-western-blot.jpg)
Western blot - Anti-CENPC antibody [EPR15939] (ab193666)Anti-CENPC antibody [EPR15939] (ab193666) at 1/1000 dilution + NIH/3T3 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 107 kDa
Observed band size: 107 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPC antibody [EPR15939] (ab193666)](https://www.abcam.com/ps/products/193/ab193666/Images/ab193666-231091-anti-cenpc-antibody-epr15939-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPC antibody [EPR15939] (ab193666)Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling CENPC with ab193666 at 1/1000 dilution (2μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-CENPC antibody [EPR15939] (ab193666)](https://www.abcam.com/ps/products/193/ab193666/Images/ab193666-231090-anti-cenpc-antibody-epr15939-flow-cytometry.jpg)
Flow Cytometry - Anti-CENPC antibody [EPR15939] (ab193666)Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling CENPC with ab193666 at 1/400 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-CENPC antibody [EPR15939] (ab193666)](https://www.abcam.com/ps/products/193/ab193666/Images/ab193666-231089-anti-cenpc-antibody-epr15939-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CENPC antibody [EPR15939] (ab193666)Western blot analysis of CENPC immunoprecipitated from Jurkat cell lysate using ab193666 at 1/100 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST. -
![Anti-CENPC antibody [EPR15939] (ab193666)](https://www.abcam.com/ps/products/193/ab193666/Images/ab193666-9-benefits-of-recombinant-antibodies.png)
Anti-CENPC antibody [EPR15939] (ab193666)
