Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)
![Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)](https://www.abcam.com/ps/products/240/ab240318/Images/ab240318-5-ab191077228170ab191077IF.jpg "Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14638] to Nkx2.2 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free
See all Nkx2.2 primary antibodies -
Description
Rabbit monoclonal [EPR14638] to Nkx2.2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab240318 is the carrier-free version of ab191077.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14638 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)
Immunofluorescent analysis of 4% paraformaldehyde-fixed C6 cells labeling Nkx2.2 with ab191077 at 1/50, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue). Negative controls also shown.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191077).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)](https://www.abcam.com/ps/products/240/ab240318/Images/ab240318-4-ab191077228169ab191077d.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191077).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)](https://www.abcam.com/ps/products/240/ab240318/Images/ab240318-3-ab191077228168ab191077c.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191077).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)](https://www.abcam.com/ps/products/240/ab240318/Images/ab240318-2-ab191077228167ab191077b.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191077).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)](https://www.abcam.com/ps/products/240/ab240318/Images/ab240318-1-ab191077228166ab191077.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191077).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)](https://www.abcam.com/ps/products/240/ab240318/Images/ab240318-6-benefits-of-recombinant-antibodies.png)
Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)
