Anti-Nkx2.2 antibody (ab93188)
Key features and details
- Rabbit polyclonal to Nkx2.2
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Nkx2.2 antibody
See all Nkx2.2 primary antibodies -
Description
Rabbit polyclonal to Nkx2.2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse Nkx2.2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab123823) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse Spinal Cord; Mouse Brain; Mouse Cerebellum; Mouse Hippocampus; Mouse Forebrain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).Target
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Function
May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. -
Sequence similarities
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Domain
The homeodomain is essential for interaction with OLIG2. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 18088 Mouse
- Entrez Gene: 366214 Rat
- SwissProt: P42586 Mouse
- Unigene: 330639 Mouse
- Unigene: 32651 Rat
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Alternative names
- Homeobox protein NK 2 homolog B antibody
- Homeobox protein NK-2 homolog B antibody
- Homeobox protein Nkx 2.2 antibody
see all
Images
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Western blot - Anti-Nkx2.2 antibody (ab93188)All lanes : Anti-Nkx2.2 antibody (ab93188) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Cerebellum Mouse Tissue Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lane 5 : Mouse Hippocampus Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Datasheets and documents
References (0)
ab93188 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Nkx2.2 antibody (ab93188)All lanes : Anti-Nkx2.2 antibody (ab93188) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Cerebellum Mouse Tissue Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lane 5 : Mouse Hippocampus Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
