Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7808] to RPS3 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RPS3 antibody [EPR7808] - BSA and Azide free
    See all RPS3 primary antibodies

  • Description

    Rabbit monoclonal [EPR7808] to RPS3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248249 is the carrier-free version of ab128995. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248249 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)
    Western blot - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)
    All lanes : Anti-RPS3 antibody [EPR7808] - Ribosome Marker (ab128995) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : Neuro-2a cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 27 kDa



    This data was developed using ab128995, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)
    Immunocytochemistry - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)
    This data was developed using ab128995, the same antibody clone in a different buffer formulation.Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma neuroblast) labeling RPS3 with ab128995 at 1/100 dilution. ab150077, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Cells were fixed with 100% Methanol. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/00 was used as counterstain. Nuclei were stained blue with DAPI.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)
    This data was developed using ab128995, the same antibody clone in a different buffer formulation.ab128995, at a 1/50 dilution, staining RPS3 in Human kidney, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)
    OI-RD Scanning - Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)
    This data was developed using ab128995, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)
    Anti-RPS3 antibody [EPR7808] - BSA and Azide free (ab248249)

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