Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab70912)
Key features and details
- Rabbit polyclonal to PI 3 Kinase catalytic subunit gamma/PI3K-gamma
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody
See all PI 3 Kinase catalytic subunit gamma/PI3K-gamma primary antibodies -
Description
Rabbit polyclonal to PI 3 Kinase catalytic subunit gamma/PI3K-gamma -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human PI 3 Kinase catalytic subunit gamma/PI3K-gamma (C terminal) conjugated to keyhole limpet haemocyanin.
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Positive control
- HeLa cell lysate; Paraffin embedded Human muscle tissue
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General notes
Previously labelled as PI 3 Kinase catalytic subunit gamma.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab70912)
ab70912, at a 1/10 dilution, staining human PI 3 Kinase catalytic subunit gamma/PI3K-gamma in muscle, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue. ab70912 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab70912)Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab70912) at 1/60 dilution + HeLa cell line lysate at 35 µg
Observed band size: 126 kDa why is the actual band size different from the predicted?
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Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab70912)All lanes : Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab70912) at 1/60 dilution
Lane 1 : Nontransfected 293 cell line lysate
Lane 2 : 293 cell line lysate transiently transfected with the PI 3 Kinase catalytic subunit gamma/PI3K-gamma gene
Lysates/proteins at 2 µg per lane.
Observed band size: 126 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa, 40 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
