Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (ab249171)
![Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (ab249171)](https://www.abcam.com/ps/products/249/ab249171/Images/ab249171-405626-anti-amps-antibody-epr10746-b-bsa-and-azide-free-western-blot.jpg "Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (ab249171)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10746(B)] to AMPS - BSA and Azide free
- Suitable for: ICC, IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free
See all AMPS primary antibodies -
Description
Rabbit monoclonal [EPR10746(B)] to AMPS - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, WBmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249171 is the carrier-free version of ab154872. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249171 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR10746(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (ab249171)All lanes : Anti-AMPS antibody [EPR10746(B)] (ab154872) at 1/1000 dilution
Lane 2 : Raw 264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HepG2 cell lysate
Lane 6 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 55 kDaThis data was developed using ab154872, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (ab249171)](https://www.abcam.com/ps/products/249/ab249171/Images/ab249171-2-anti-amps-antibody-epr10746-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (ab249171) -
![Immunoprecipitation - Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (ab249171)](https://www.abcam.com/ps/products/249/ab249171/Images/ab249171-399572-anti-amps-antibody-epr10746-b-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (ab249171)This data was developed using ab154872, the same antibody clone in a different buffer formulation.
AMPS was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell extract with ab154872 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab154872 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.
Lane 1: NIH/3T3 whole cell extract, 10 µg (Input).
Lane 2: ab154872 IP in NIH/3T3 whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab154872 in NIH/3T3 whole cell extract.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 1 second.
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![Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (ab249171)](https://www.abcam.com/ps/products/249/ab249171/Images/ab249171-4-benefits-of-recombinant-antibodies.png)
Anti-AMPS antibody [EPR10746(B)] - BSA and Azide free (ab249171)