Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7465] to Fatty Acid Synthase
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Fatty Acid Synthase antibody [EPR7465]
    See all Fatty Acid Synthase primary antibodies

  • Description

    Rabbit monoclonal [EPR7465] to Fatty Acid Synthase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Fatty Acid Synthase aa 1-100. The exact sequence is proprietary.
    Database link: P49327

  • Positive control

    • WB: HeLa, 293T, A549, SHSY-5Y and MOLT4 cell lysates. ICC/IF: A549 cells. IHC-Fr: Human peritoneal tumor tissue. Flow Cytometry: A549 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Images

  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg)
    Lane 3: A549 cell lysate (20 µg)
    Lane 4: Hu liver tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab128856 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab128856 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab128856 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    All lanes : Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : SHSY-5Y cell lysate
    Lane 5 : MOLT4 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 273 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    ab128856 at a 1/250 dilution staining Fatty Acid Synthase in A549 cells by immunofluorescence.
  • Flow Cytometry - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Flow Cytometry - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Overlay histogram showing A549 cells stained with ab128856 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128856, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • OI-RD Scanning - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    OI-RD Scanning - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)

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