Anti-CEBP Alpha/CEBPA antibody (ab140479)
Key features and details
- Rabbit polyclonal to CEBP Alpha/CEBPA
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CEBP Alpha/CEBPA antibody
See all CEBP Alpha/CEBPA primary antibodies -
Description
Rabbit polyclonal to CEBP Alpha/CEBPA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Chimpanzee, Macaque monkey
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Immunogen
Synthetic peptide corresponding to Human CEBP Alpha/CEBPA aa 250-350 conjugated to keyhole limpet haemocyanin.
Database link: P49715 -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Kidney, Mouse Kidney, Human Pancreas, Human Liver. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas.
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General notes
This product was previously labelled as CEBP Alpha
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab140479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 38 kDa). IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Target
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Function
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. -
Sequence similarities
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 281677 Cow
- Entrez Gene: 1050 Human
- Entrez Gene: 12606 Mouse
- Entrez Gene: 24252 Rat
- Omim: 116897 Human
- SwissProt: O02754 Cow
- SwissProt: P49715 Human
- SwissProt: P53566 Mouse
see all -
Alternative names
- Apoptotic cysteine protease antibody
- Apoptotic protease Mch 5 antibody
- C/EBP alpha antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody (ab140479)
IHC image of CEBP Alpha/CEBPA staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab140479, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-CEBP Alpha/CEBPA antibody (ab140479)All lanes : Anti-CEBP Alpha/CEBPA antibody (ab140479) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Kidney (Mouse) Tissue Lysate (ab27254)
Lane 3 : Human pancreas tissue lysate - total protein (ab29816)
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa (possible non-specific binding), 53 kDa (possible non-specific binding)
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140479 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
References (1)
ab140479 has been referenced in 1 publication.
- Li M et al. MIR221HG Is a Novel Long Noncoding RNA that Inhibits Bovine Adipocyte Differentiation. Genes (Basel) 11:N/A (2019). PubMed: 31887993
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody (ab140479)
IHC image of CEBP Alpha/CEBPA staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab140479, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-CEBP Alpha/CEBPA antibody (ab140479)All lanes : Anti-CEBP Alpha/CEBPA antibody (ab140479) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Kidney (Mouse) Tissue Lysate (ab27254)
Lane 3 : Human pancreas tissue lysate - total protein (ab29816)
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa (possible non-specific binding), 53 kDa (possible non-specific binding)
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140479 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
